Human and animal cell culture applications in biotechnology, medicine, and tissue engineering. Ex vivo expansion and controlled differentiation of hematopoietic (blood) stem and progenitor cells for use in transplantation therapies, with a focus on megakaryocytic cells, platelets and immunomodulatory cells. Development of culture surfaces that mimic aspects of the in vivo bone marrow niche to increase stem cell expansion or modulate differentiation. Analysis of mechanisms that regulate hematopoietic cell differentiation. Development of bioreactor systems for hematopoietic cell and platelet production and also to support the recellularization of decellularized organs.
Overview of the Differentiation of Hematopoietic Stem Cells to Megakaryocytes and Platelets
Platelet Production in Culture
"Three-Stage Ex Vivo Expansion of High-Ploidy Megakaryocytic Cells: Towards Large-Scale Platelet Production," S. Panuganti*, A.C. Schlinker*, P.F. Lindholm, E.T. Papoutsakis, and W.M. Miller, Tissue Eng. A, 19: 998-1014 (2013). PMCID: PMC3592379.